ABSTRACT
Genome annotation projects can produce incorrect results if they are based on obsolete data or inappropriate models. We have developed an automatic re-annotation system that uses agents to perform repetitive tasks and reports the results to the user. These tasks involve BLAST searches on biological databases (GenBank) and the use of detection tools (Genemark and Glimmer) to identify new open reading frames. Several agents execute these tools and combine their results to produce a list of open reading frames that is sent back to the user. Our goal was to reduce the manual work, executing most tasks automatically by computational tools. A prototype was implemented and validated using Mycoplasma pneumoniae and Haemophilus influenzae original annotated genomes. The results reported by the system identify most of new features present in the re-annotated versions of these genomes.
Subject(s)
Databases, Genetic , Computational Biology/methods , Genome, Bacterial , Haemophilus influenzae/genetics , Mycoplasma pneumoniae/genetics , Software , Open Reading Frames/genetics , Database Management SystemsABSTRACT
Os autores apresentam um caso de adenoma tubular apócrino, com alteraçöes histopatológicas particulares associando imagens de seiringoma de tricoepitelioma e de adenoma infundibular
Subject(s)
Middle Aged , Humans , Male , Female , Adenoma/pathology , Sweat Gland Neoplasms/pathologyABSTRACT
Os autores apresentam um caso de cisto triquilemal proliferante de Wilson-Jones, em um paciente de 63 anos, localizado na regiäo parieto-occipital esquerda. Os aspectos clínico-histopatológicos e histogenéticos do tumor säo discutidos. Chamam atençäo para as semelhanças histológicas com o epitelioma espinocelular
Subject(s)
Middle Aged , Humans , Male , Epidermal Cyst/pathology , ScalpSubject(s)
Humans , Bacterial Infections , Calymmatobacterium , Streptomycin , Diagnosis, Differential , Socioeconomic FactorsABSTRACT
Foi nossa proposta a etiologia controvertida do eritema indurado de Bazin e, para isto, realizamos revisao do material existente nos Servicos de Dermatologia da Policlinica Geral do Rio de Janeiro, da Santa Casa de Misericordia, da Universidade Federal do Rio de Janeiro de da Universidade Federal Fluminense. Incluimos no nosso material o metodo tintorial proposto por NYKA, a fim de comprovar sua sensibilidade na evidenciacao de mycobacterias.Os criterios de tuberculose foram revistos e comparados com aqueles adotados para o diagnostico de outras infeccoes principalmente da hanseniase. Os criterios presuntivos de diagnostico da tuberculose cutanea propostos por F.E. Rabello foram analisados e aplicados a nossa causistica